softWoRx provides tools that you can use to acquire, process, visualize, and analyze multi-dimensional renderings of a fluorescent specimen. It also allows you to save data in a variety of formats.
You can use softWoRx to acquire images or to import images in other formats.
Acquire images with the DeltaVision Imaging System.
Import images from the TIFF format(8-bit or 16-bit grayscale), BioRad’s PIC format, InoVision’s ISee format, or MetaMorph’s STK format.
Use tools to correct, deconvolve, filter, and stitch images:
Correct data collection errors and equalize intensity values across Z sections.
Deconvolve images to improve contrast by relocating signal scatter and out-of-focus information.
Correct chromatic aberration (i.e., shifts in color that result from oil matching and other environmental conditions).
Filter data to prepare for modeling or other types of analysis.
Stitch images together that are organized as either a series of time points or Z sections.
After processing data, you can view it in a variety of ways:
Adjust image displays (e.g., display a scale bar, display in grayscale or color modes, and adjust brightness and contrast).
View movies of volume-rendered data or time-lapse data.
Project 3D images onto a 2D plane.
Adjust the colors of the final images.
Rotate and resection volumes of data.
Combine image data together.
Render volumetric views of images for visual exploration of the data.
You can use measuring and modeling tools to perform quantitative analysis:
Examine intensity data to study area and line profiles, calculate statistics, and display single point values.
Measure features with interactive measuring tools, including distance measurement and point recording.
Use Colocalization to create a scatter plot of results and measure the Pearson Coefficient of Correlation.
Calculate Fluorescence Resonance Energy Transfer (FRET) and net FRET.
Analyze Fluorescence Recovery After Photo Bleaching (FRAP) experiments.
Use modeling tools to create and display 3-D models of features in the image data and to extract quantitative data.
Export images for viewing in graphic applications such as PhotoShop or save them as MPEG or QuickTime movies.
Archive the data to a CD-ROM.
Save image data in data files or in a tabular format that can be opened in a spreadsheet.