DeltaVision’s live cell imaging method allows you to capture time-lapse images of multiple points (in different fields of view) in a single sample. Because you only have to prepare your sample once, this method significantly reduces the time required to prepare and conduct your experiments.
DeltaVision significantly reduces the lab time required to perform time-course studies of genetically, biochemically, and bio-mechanically manipulated cells. You can prepare one sample and capture time-lapse images of several cells in one imaging session instead of preparing multiple samples and capturing time-lapse images of only one cell in each sample. You can also improve the odds of identifying and monitoring surviving cells in experiments that typically have high cell mortality rates by monitoring a large set of samples.
Use the Resolve3D module to acquire live cell data as follows:
Set up Acquisition Parameters: Use the Settings window to set file output directories and to select camera settings for your experiment.
Acquire an Image: Use the joystick and the Resolve3D window to find the specimen, set the exposure time, and find the specimen thickness. Then acquire images manually and adjust these parameters until you are satisfied with the quality of the image.
Design a Time-Lapse Experiment: Find and mark the points that you want to monitor in the sample. Then set up the time-lapse intervals for the experiment.
To find and center the specimen