To align images:

Use Align Image to correct motion artifacts, problems with Z sectioning, or problems with time series. This tool allows you to align adjacent images by applying an XY shift with an optional rotation. Use Align Image only for images that have a single wavelength.

  1. From the main softWoRx menu, choose Process | Align Image to open the Align Image window.

  2. Click Input and browse to the appropriate file. The Range fields and Wavelengths are filled in automatically when a file is selected.

    Note:  This window requires an image name for processing. You cannot provide a window number for this field.

  3. In the Align Adjacent list, select whether to align adjacent Z Sections, Wavelengths, or Time Points.

  4. Click Do It to align the image.