You can calibrate a raw image when you have a calibration file and a bad pixel file that applies to the camera and conditions (array size, pixel size, and wavelength) used to collect this image. If you do not have a calibration file, you must create one before you calibrate the image.
From the main softWoRx menu, choose Process | Calibrate to open the Calibrate window.
Click Input and browse to the file that you want to calibrate.
To select a region of the file, click Details and use the Region Details window to select the region.
Click the Cal button and browse to the calibration file to use for this image.
Click the Pix button and browse to the bad pixel file to use for this image.
Select the types of calibration to perform (Calibrate Gain, Calibrate Offset, or Replace Bad Pixels).
Click Do It to calibrate the image.