Calibrate Resolve3D when you are interested in very low intensity changes (1-20 counts) on the camera or when you are unsatisfied with the uniformity across the camera field of view.
During image acquisition with a calibrated DeltaVision system, the CCD camera response is adjusted according to the slope and offset information in the calibration table. Because the calibration is applied as the image data is acquired, calibrated DeltaVision systems produce images that are much better than those produced by uncalibrated systems. Calibration is especially useful when imaging weak fluorescence signals, because calibration improves the signal-to-noise ratio within the collected images.
By calibrating images acquired with Resolve3D, you can simultaneously compensate for a non-uniform field of illumination and non-uniform CCD detector response. The calibration process has the following goals:
Determine the sensitivity of each pixel to a range of light intensities. By comparing the actual response to the ideal response, a correction factor can be determined for each pixel.
Determine the offset of each pixel. The base value of each pixel can be adjusted so that it is nearly equal to all other pixels in the image.