When you press the Start Acquiring Images button on the Continuous Acquire window, the camera collects images using the excitation and exposure settings currently selected in the Resolve3D main menu.
From the Continuous Acquire window, you can adjust the following focus parameters:
The Maintain every ____ frames field defines how often the stage corrections for UltimateFocus are made. Entering 1 tells softWoRx to perform the Move Threshold check for every image, entering 2 indicates performing the process for every other image acquired, and so on. Entering a higher number in this field will increase the speed of your acquisitions.
The Move Threshold field defines the minimum Z position change from the point of origin. If the change in the Z position is less than the value indicated in the Move Threshold field, the position of the stage will not change. If the change in position is greater than the Move Threshold, the stage will move to correct the Z position. The default value is set to 0.100 microns.
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Note: For experiments in which fast acquisition speed is the most important factor, limiting the number of corrections will increase the speed of the image acquisition.. |
The Max Iterations field defines the number of stage corrections the UltimateFocus module will make using the Move Threshold. This field sets the upper limit for corrective moves allowed to the stage position at one time. Limiting the number of iterations increases the speed of your image acquisitions at the possible cost of focus accuracy. For fast imaging, it is recommended that the setting in the Max Iterations field be set to no greater than 1.