To import Inovision ISeeTM files:

  1. On the softWoRx main menu, choose Conversions | Import from ISee to open the Convert InoVision ISee to DeltaVision window.

  2. In the ISee File field, enter the file name of the Inovision ISee image file.

  3. In the DV File field, enter the file name for the DeltaVision image file.

  4. To combine all files with a similar name into one DeltaVision file, select Convert Series.

  5. In the Wavelength field, enter the wavelength of the light collected by the camera. For fluorescence images, use the wavelength of the emission filter.

  6. In the X Spacing, Y Spacing, and Z Step Size fields, enter the X, Y, and Z spacing of the pixels in the data set. If 0 is entered in these fields, the program to uses the corresponding spacing found in the Inovision ISee image file.

  7. In the Lens ID field, enter the DeltaVision lens ID number. (See Lens Information.)

  8. To change the default directory or file name for the command file or the log file, click Options.

  9. Click Do It.

Notes

  • The deconvolution program and other softWoRx software rely upon the presence of accurate wavelength and pixel spacing. Because not all ISee files contain accurate pixel size and wavelength, you may need to manually enter some of the fields. Image wavelength for a DeltaVision file usually indicates the wavelength of the light imaged by the camera, rather than the illumination wavelength.

  • The X,Y pixel spacing can be obtained in two ways: it can be measured with a test target, or it can be approximated from the CCD detector element size and the total image magnification.

  • The conversion program combines files that have the same prefix as the input file. It assumes that a series of Inovision files have the same name, except for the sequence numbers in the last 3 characters. For example, the my_file_name.001, my_file_name.002, and my_file_name.003 files are automatically combined into a single DeltaVision file. You can enter any of these files in the ISee File field. The use of wildcards is not supported.

    For example, if the CCD detector has 6.7 um pixels and the image was acquired with a 100X lens AND a 1.5X optivar, then the pixel size is approximately 6.7um/(100 x 1.5) = 0.045 um. The Z pixel spacing is the distance between adjacent optical sections.