Use the PSF to OTF program to convert a measured point spread (PSF) to an optical transfer function (OTF). The OTF is the Fourier transform of the PSF. The pixel size of the resulting image is given in cycles/micrometer. To help reduce problems associated with measurement noise, the PSF is radially averaged during the conversion. As a result, the 3D PSF image becomes a 2D OTF image.
The horizontal axis of the OTF represents radial (XY) frequency, whereas the vertical axis represents axial (Z) frequency. The brightness of the OTF image elements, on a scale of 0 to 1, represents the frequency response of the microscope system at the corresponding radial and axial frequencies.
Open the PSF to OTF Conversion window by choosing Conversions | Convert PSF to OTF from the main sotWoRx menu.
Use |
To... |
PSF File |
Browse to the name of the PSF image file that you want to convert to an OTF. |
OTF File |
The OTF file name is automatically created by appending _otf to the PSF file. Axial symmetry is assumed, so that the resulting OTF contains only the magnitude of the complex OTF. |
X, Y, and Z Range |
Specify the central XYZ coordinates of the point spread. |
T Range |
Specify the time range. |
Wavelengths |
Specify the wavelength at which the PSF was collected. |
Center |
Specify the XYZ image dimensions about the central coordinates. Note that the standard DeltaVision softWoRx point spread measurement is 256x256x128. |
Size |
Specify the XYZ dimensions about the central coordinates.
Note: The standard DeltaVision point spread measurement is 256x256x128. |
Lens ID |
View the lens identification number of the PSF file. |
Additional Parameters |
Enter the number of voxels to roll-off at the edge of the image. Border roll-off reduces edge effects resulting from the Fourier transform. |
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