To import BioRad Pic (TM) image files:

Choose Conversions | Import from PIC on the softWoRx main menu.
In the Pic File field, enter the file name of the Pic image file.
In the DV File field, enter the file name for the DeltaVision image file.
If the image file consists of two wavelengths that are arranged in two adjacent panels, select Split Adjacent Panels. (This superimposes the two wavelengths into a DeltaVision image.)
In the Wavelength 1 field, enter the wavelength of the first image (or panel).
If the image has two wavelengths, enter the wavelength of the second panel in the Wavelength 2 field.
In the X Spacing, Y Spacing, and Z Step Size fields, enter the X, Y, and Z spacing of the pixels in the data set.
In the Lens ID field, enter the DeltaVision lens ID number. (See Lens Information.)
Click Do It.

Notes

Not all PIC files contain accurate pixel size and wavelength, so it may be necessary to manually enter some of the fields described below. Note that the deconvolution program and other softWoRx software relies upon the presence of accurate wavelength and pixel spacing.
Image wavelength for a DeltaVision file usually indicates the wavelength of the light imaged by the camera, rather than the illumination wavelength. The X,Y pixel spacing can be obtained in either of two ways: it can be measured with a test target or it can be approximated from the CCD detector element size and the total image magnification. For example, if the CCD detector has 6.7 um pixels and the image was acquired with a 100X lens AND a 1.5X optivar, then the pixel size is approximately 6.7um/(100 x 1.5) = 0.045 um. The Z pixel spacing is the distance between adjacent optical sections.

Not all PIC files contain accurate pixel size and wavelength, so it may be necessary to manually enter some of the fields described below. Note that the deconvolution program and other softWoRx software relies upon the presence of accurate wavelength and pixel spacing.