From the softWoRx main menu, choose Conversions | Import from MetaMorph STK to open the Convert MetaMorph STK to DV window.
In the STK File field, enter the file name of the MetaMorph STK image file.
In the DV File field, enter the file name for the DeltaVision image file.
In the Stack Type field, specify whether the input data set is a Z Series or a Time Lapse stack.
In the Wavelengths field, enter the wavelength of the light collected by the camera. For fluorescence images, use the wavelength of the emission filter.
In the X Spacing, Y Spacing, and Z Step Size fields, enter the X, Y, and Z spacing of the pixels in the data set (see Note #2 below). The program reads these values from the STK file.
in the Lens ID field, enter the DeltaVision lens ID number. (See Lens Information.)
To convert the data types to signed 16 bit integers, select Convert to signed 16 bit. (DeltaVision files normally store data in signed 16 bit integers.)
Click Do It.
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Note #1 Not all STK files contain accurate pixel size and wavelength information, so it may be necessary to manually enter some of the fields. When you specify the input file, the conversion program attempts to read the wavelength and pixel size values from the STK file's header. The deconvolution program and other softWoRx modules rely upon the presence of accurate wavelength and pixel spacing. The image wavelength for a DeltaVision file usually indicates the wavelength of the light imaged by the camera, rather than the illumination wavelength. #2 The X,Y pixel spacing can be obtained in either of two ways: it can be measured with a test target, or it can be approximated from the CCD detector element size and the total image magnification. For example, if the CCD detector has 6.7 um pixels and the image was acquired with a 100X lens AND a 1.5X optivar, then the pixel size is approximately 6.7um/(100 x 1.5) = 0.045 um. The Z pixel spacing is the distance between adjacent optical sections. |