softWoRx provides several tools for correcting image data:
Correct Image is used to correct errors are caused by photo-bleaching, inconsistent illumination intensity, and CCD defects. By default, softWoRx automatically applies this tool during the deconvolution process. The Correct Image tool corrects the intensity values of sections within a Z series. With the exception of photo-bleaching, the tool can also correct intensity values between time points of a time-lapse experiment.
Equalize Time Points equalizes intensities of all time points to a reference time point that you select. The tool uses the mean image intensity to help generate a more uniform intensity display. You can use this tool to normalize time-lapse data for display purposes. In general, the data generated by this tool should be used for display only and should not be used for quantitative purposes.
Calibrate calibrates a raw image when you have a calibration file and a bad pixel file that applies to the camera and conditions (array size, pixel size, and wavelength) used to collect the image.
Align Image corrects single wavelength images that have motion artifacts, problems with Z sectioning, or problems with time series. It allows you to align adjacent images by applying an XY shift with an optional rotation.
Chromatic Aberration Corrector to adjust channels relative to each other to correct for shifts in color that result from oil matching and other environmental conditions that use X-Z and Y-Z image profiles.