You can create line models that trace objects such as chromosomes or neurons through 3D space. With these models, you can extract data from images to perform 3D karyotyping. Use the following process to create line models:
Prepare Data:Before you trace features in 3D and extract linear models, you may need to use the tools in the Filter menu to prepare the images by filtering, creating thresholds, or enhancing contrast.
Trace Features in 3D: Use the 3D Model tool to manually or automatically trace model objects. You can also trace features in an image using the AutoModel function of 3D Model. AutoModel takes a starting point that you specify and searches through the image volume for a path that is constrained by parameters that you can control. You can choose to have AutoModel look for the entire object in one continuous search, or do a stepwise search that allows you to intervene to get through areas of noisy data.
Apply the Model: Use Line models to extract information from the image data. The Straight tool takes Line models, extracts tubes of data from the image, and displays the results in various ways. If applied to chromosomes, this may be a way to do 3D karyotyping.