Localization Phase - Identify
and localize fluorophores within the acquired data.Analysis of localization microscopy data occurs in the following four phases:
Localization Phase - Identify
and localize fluorophores within the acquired data.
Correction
Phase – Correct fluorophore positions to account for lateral
stage drift during data acquisition. Drift is measured using an image
correlation algorithm and does not require the use of fiducial markers.
Tracking
Phase - Track fluorophores that appear in more than one frame.
This phase can also be used to remove lateral drift by tracking fiducial
markers.
Reconstruction Phase - Generate
a super-resolution DeltaVision image.
The tools for setting up each of these phases are available in the Localization Image Analysis window.
Localization Phase Input Parameters
Setting Up Image Correlation Drift Correction
Setting Up Image Reconstruction