The Data Inspector window displays image intensity and intensity ratio data from a region of interest (ROI) in the Image window in various formats. Using Data Inspector, you can define the size of the region of interest and save data to ASCII files.
Open the Data Inspector window by choosing Tools | Data Inspector on the Image window menu.
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Use |
To... |
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File Menu |
Save the currently viewed table of data in an ASCII text file for off-line processing, open a file to collect statistics records, or close the Data Inspector window. |
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View Menu |
Create a 3D graph or a Histogram display. |
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Options Menu
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Choose options for the format of the statistics record output file, select options for subtracting background intensity from calculations, choose the wavelengths to use in ratio calculations, and modify settings (number of bins, bin width, etc.) for histogram plots. |
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Current Window |
Enter the window number of the Image window from which data is being displayed in the Data Inspector window. |
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Select Box |
Define a region of interest (ROI) in the Image window. Click Select Box and then move the cursor to the Image window and select a region to define the ROI. The data from this ROI is then displayed by the Data Inspector window, whether in the Data Table, Histogram or 3D Data Graph. |
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Columns/Rows |
Define the size of the region of interest. Columns is the number of horizontal pixels, and Rows is the number of vertical pixels in the region of interest. You can define the size by changing the values in the fields, or by clicking Select Box and defining the ROI graphically. |
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Constrain to Circle |
Display intensity data for pixels inside the circle shown by the ROI box in the Image window. This makes it possible to display data for the pixels in a spot without including background pixels. |
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Show 3D Graph |
Open a 3-D graph of ROI pixel intensities in a separate window. The graph updates automatically as you move and resize the ROI. |
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Show Histogram |
Display a histogram of the intensity distribution of pixels in the ROI in a separate window. The graph updates automatically as you move and resize the ROI. |
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Ratio |
Change the values displayed in the table, 3D graph, and histogram from absolute intensity values to intensity ratios of channels in the ROI. |
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Show Ratio Graph |
Open the Ratio Graph. (This is active only when Ratio is selected.) |
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Statistics |
Display the minimum, maximum, and mean intensities, number of pixels, and the standard deviation of intensity values of the image file that is displayed in the Image window. If Ratio is selected, the Statistics field displays the average ratio of intensities in the ROI. |
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Data Table
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Display intensity values of each pixel in the region of interest. If Ratio is selected, the table displays the intensity ratio of two channels for each pixel (e.g., Cy3:Cy5). |
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