To deconvolve an image with the Constrained Iterative tool:
You can deconvolve images that are acquired with the DeltaVision system.
From the softWoRx main menu, choose Process | Deconvolve to open the Deconvolve window.
In the Input field, enter the original .r3d image file.
softWoRx creates an output file name by appending the _d3d extension to the input file name. The new name is displayed in the Output field.
In the OTF File field, enter the .otf file. For DeltaVision files, this is done automatically. ( In many microscopy systems, there is only one OTF per objective lens and the correct OTF is simply the one that corresponds to the lens used for measuring the optical sections. Refer to the lens identification number in the measured data and the OTF to verify that the correct OTF is being used.)
To specify a deconvolution algorithm, select one in the Method list. (The default algorithm is adequate for most applications.)
To adjust the level of Gaussian filtering, choose a filtering level in the Noise Filtering list.
Click Do It.
The deconvolution status is displayed in the Deconvolve Output window and on the bottom of the Deconvolve window. When the deconvolution is finished, messages appear in each of these windows.
From the softWoRx menu, choose File | Open to open the Open Image window.
Browse to and select the new .DV file..
Click OK to open the file in the Image window and make sure that the image quality is good.