Constrained Iterative Deconvolution Options

Use

To...

Input, Output, Select Region, Reset, Details,

Wavelengths

Specify input data and output parameters as shown in Image Input and Output.

OTF File

Enter the name of the OTF file used to deconvolve the image. This filename is typically automatically identified by softWoRx. 

Note When a new image is selected for deconvolution, an OTF file name is automatically generated, based on the lens ID of the image file. If the lens ID matches one of the OTFs listed in the system configuration files, that OTF is used by default. Otherwise, the appropriate OTF file name is automatically generated from the lens ID. 

Method

Choose one of the following deconvolution methods:

Ratio (the default) is a more conservative algorithm than enhanced ratio and converges more reliably on a solution. Images with punctate fluorescence deconvolve better with the Ratio method.

Enhanced Ratio provides faster convergence.

Additive is the best method for data acquired with the EMCCD electron multiphoton camera. This method is tolerant of data with a low signal-to-noise ratio.

Enhanced Additive is similar to Additive. This method is faster than additive because it requires fewer iterations (typically ten or fewer).  

Number of Cycles

Specify the number of deconvolution iterations. For shorter deconvolution times, try setting this value to about 5 or 10. (At least 4 iterations is recommended.) For higher resolution (that is, finer detail) try increasing the number of iterations. The default number of iterations is 15.

Noise Filtering

Choose the level of Gaussian filtering. Each of these options sets the Prefilter Resolution Limits for the Gaussian pre-filter.

Apply Correction

Apply image correction procedures before deconvolution.

Deconvolve Projections

This option is used only when the image is a projection of the measured image. This is NOT the same as deconvolving a single Z section. That is, you should not use this option when deconvolving images with only one Z section. The default is off, except when the input image is a projection image.

Run Options

Specify options for running the deconvolution process. The following options are available:

• Log File specifies the name of a file that holds an informational record of the deconvolution process.

• Command File specifies the name of the command shell script that is used to run the process.

• Run Options allows you to choose from three options that specify when the job will run. Run Now runs the job immediately after choosing the Do It button. Run Later creates a command shell script that is manually executed at a later time. Add To Queue puts the job at the end of a list of commands to be executed in order by the Queue Manager.

• Run at Low Priority allows you to use the workstation for other tasks while the deconvolution is performed.

• Show Output Log opens a window that shows the progress of the process.