Use Equalize Time Points to choose a reference time point and equalize intensities of all other time points to it. (The Correct tool and the Correction options of the Deconvolve tool make corrections to the intensity values of sections within a Z series and, with the exception of photo-bleaching inconsistencies, can also make corrections to intensity values between time points of a time-lapse experiment.)
From the main softWoRx menu, choose Process | Equalize Time Points to open the Equalize Time Points window.
In the Input field, enter the original .r3d image file.
In the Reference Time Point field, enter the time point to use as the standard for adjusting intensity values of other time points.
To perform equalization based on background and minimize the influence of the variation of higher intensities (signal), select Use Threshold. (This specifies to use only values less than the Threshold value for each wavelength when collecting Min/Max/Mean statistics for equalization.)
If you are using a threshold, enter the threshold for each wavelength in the Threshold field.
Click Do It to equalize the time points and then click Done.
Tip: If you are using a threshold, you can view the maximum, minimum, and mean values for each wavelength by clicking the Wave button. |