1. From the Resolve3D
main menu, select the Microtiter Plate
icon. A representation of
the microtiter plate is displayed in the Plate View window.
2. From the Plate Geometry Overlay field, select the plate geometry that corresponds to the plate you have loaded into the system for this experiment.

3. Click the Visit
icon to put the Plate View window in Visit mode.
4. Click on a well to visit the nominal X/Y center of the well.
5. Perform a rough focus on the plate with the eyepieces and the microscope’s focus knobs.
6. Click the Autofocus
icon from the Resolve3D window to activate Autofocus.
7. Explore the plate to set up filters, exposure times, binning, and the rest of the variables you would normally configure for an acquisition.