From the softWoRx main menu, choose Filter | Filter2D to open the 2D Filter window.
In the Input field, enter an image file name or window number.
To include only selected data, click Details to open the Region Details window. Then specify the ranges of data that you want to include in the Output Options fields.
Select which wavelengths to filter.
In the Method option list, select one of the following filters:
Median uses the image intensities within a box-shaped region around each pixel and selects the median value for the resulting image.(This is useful for removing noise.)
Mean is similar to the median filter except that the mean value is used instead of the median.
Variance calculates the statistical variance of the image about the mean intensity within the local region.
weighted Mean is similar to the mean filter, except that mean is weighted by the variance.
In the Kernel Size field, specify the size ( in pixels) of the square box used to calculate the filtered images. (Kernel sizes of 3 and 5 are most useful.)
In the Iterations field, specify the number of times to run the filter.
Click Do It.
The filtered image is displayed in a new image window.