Depending on which broadband light source the system uses, the DeltaVision imaging system provides a variety of different filter sets for both fixed and live cell imaging. These filters are designed to be used with many common fluorescent probes. If you are using fluorescent probes that are not well matched with the filters you have, contact GE Healthcare for assistance. (For instructions that show how to swap filter modules, see the DeltaVision Imaging System User's Manual.)
The 4-color Fixed InsightSSI module includes four of the most common sets of filters used for fixed-cell imaging:
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InsightSSI 4-color Fixed |
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FilterName |
Excitation |
Emission |
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DAPI |
UV, 381-401nm |
Blue, 409-456nm |
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FITC |
Blue Green, 464-492nm |
Green, 500-523nm |
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TRITC |
Yellow Green, 531-556nm |
Yellow, 564-611nm |
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CY-5 |
Red, 619-644nm |
Infrared, 652-700nm |
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The 4-color Live Cell InsightSSI module includes four of the most common sets of filters used for live-cell imaging:
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InsightSSI 4-color Live Cell |
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FilterName |
Excitation |
Emission |
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CFP |
Blue, 400-454nm |
Blue Green, 463-487nm |
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GFP |
Blue Green 425-495nm |
Green, 500-550nm |
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YFP |
Green, 496-528nm |
Yellow Green, 537-559nm |
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mCherry |
Yellow, 555-590nm |
Orange, 600-675nm |
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The 7-color Live Cell InsightSSI includes seven of the most common sets of filters used for fixed and live cell imaging:
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InsightSSI 7-color (for Fixed and Live Cell Imaging) |
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FilterName |
Excitation |
Emission |
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DAPI |
UV, 381-401nm |
Blue, 409-456nm |
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CFP |
Blue, 400-454nm |
Blue Green, 463-487nm |
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FITC-GFP |
Blue Green 425-495nm |
Green, 500-550nm |
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YFP |
Green, 496-528nm |
Yellow Green, 537-559nm |
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TRITC |
Yellow Green, 531-556nm |
Yellow, 564-611nm |
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mCherry |
Yellow, 555-590nm |
Orange, 600-675nm |
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CY-5 |
Red, 619-644nm |
Infrared, 652-700nm |
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The excitation and emission peaks of the Fixed Cell filters for a DeltaVision system using a xenon arc lamp for its broadband light source are provided in the following table:
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Xenon Fixed Cell Filter Set |
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FilterName |
Excitation |
Emission |
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DAPI |
UV, 325-375nm |
Blue, 438-478nm |
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FITC |
Blue Green, 481-502nm |
Green, 506-543nm |
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TRITC |
Green, 547-563nm |
Yellow/Orange, 576-630nm |
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CY-5 |
Red, 636-656nm |
Infrared, 667-719nm |
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The excitation and emission peaks of the Live Cell filters for a DeltaVision system using a xenon arc lamp for its broadband light source are provided in the following table:
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Xenon Live Cell Filter Set |
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FilterName |
Excitation |
Emission |
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CFP |
Blue, 415-445nm |
Blue Green, 455-485nm |
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YFP |
Green, 490-510nm |
Yellow Green, 520-550nm |
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mCherry |
Yellow, 555-590nm |
Orange, 600-675nm |
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GFP |
Blue Green, 425-495nm |
Green, 500-530nm |
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