Use options on the Resolve3D Settings Illumination tab to set up illumination parameters.
Open the Illumination settings options by clicking the Settings
icon on the Resolve3D window and clicking the Illumination
tab.
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To... |
Field Stop Aperture (Closed <-> Open) |
This field allows you to set the field stop aperture to Closed, Open, or any percentage in between. You can either enter a percentage in the field or use the slider to set the percentage. |
Critical/Köhler Illumination Switching |
On an GE Healthcare Fluorescence Illumination Module, there are three choices for the motorized Critical/Köhler illumination position: Automatic – DeltaVision automatically adjusts the position of the critical/Köhler illumination when the EX shutter is opened using the keypad (typically when the eyepieces are being used). The position is automatically returned to critical illumination when an image is requested, either via Resolve3D or by running an experiment. Always Critical – DeltaVision moves the critical/Köhler motor to critical illumination and leaves it in its position regardless of the current shutter states. Always Köhler – DeltaVision moves the critical/Köhler motor to Köhler illumination and leaves it in its position regardless of any new image requests or experiments running. |
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