- For short time-lapse intervals that are safely greater than the imaging time requirement.
In this scenario, you can specify Maintain Focus with UltimateFocus in the Time-lapse tab of the Experiment Designer. When you encounter this situation while running the experiment, the system will determine whether it has enough time to do the UltimateFocus maintenance and if so, do it just before the time lapse expires and imaging starts. The idea is to get focus adjusted as close to the start of imaging as possible.