Open an image in the Image window. From the Image window menu, choose Tools | Line Profile.
Drag the window number from the Image window to the Window field in the Line Profile window.
Click on the image to display a horizontal line profile.
To get the average line profile of several rows and columns of pixels, enter the width in the Band field.
To display a vertical line profile, choose Vertical in the Direction list.
To change the position of
the profile, click another point on the image or use the 
and 
next to the XY field.
Create a group of profiles to save in a file as follows:
To save only selected profiles, deselect AutoSave. Then click Save
after each profile that you want to save is displayed.
-or-
To save all profiles, select AutoSave before you create the profiles.
To save a group of profiles, click Write to File, enter the file name and other options in the Profile Output Options window, and click Do It.