AutoFocus Action Options

Use the AutoFocus action to set up autofocus parameters for a time-lapse experiment.

1. From the Actions tab on the Design tab, select AutoFocus as the action and then select Options. The AutoFocus Action Options window is displayed.

2. To have the options in this window set automatically, activate the Auto-Determine Parameters check box. Then click the Done button. If you do not activate the Auto-Determine Parameters check box, set the remaining fields in the window as described in the following steps.

3. Use the drop-down list in the Channel for Autofocus field to select the channel you want to use for Autofocus.

4. Use the drop-down list in the Contrast calculation method field to select Auto, Fluorescence (Light on Dark), or Brightfield (Dark on Light).

5. Select the Autofocus Z test step for this experiment.

6. Choose the Maximum Z test range for this experiment.

7. Indicate any Post-autofocus Z offset for this experiment.

8. Click the Done button.