Laser Action Options

Use the Laser Event action to set up laser events for a time-lapse experiment.

1. From the Actions tab on the Design tab, select Laser Event as the action and then select Options. The Laser Action Options window is displayed.

2. On the Laser Action Options window, use the drop-down list in the Laser focus point field to select Middle of Sample, Bottom of Sample, or Top of Sample.

3. Select the Laser channel to use and specify the Duration (in seconds), or use the Refresh button to update the active laser and duration to that currently used in the Laser Module Settings window.

4. Select how many laser events you want for this action in the Number of events field.

5. Use the drop-down list in the Bleach event motion field to select either Optimize for Position or Optimize for Speed.

6. Use the drop-down list in the Event pattern field to select either Use Current Center Point or Use Bleach Event Specification.

7. If you selected Use Bleach Event Specification in the previous step, use the now active tools in the Create Bleach Events section of the window to generate specific bleach events, otherwise, skip to the next step.

8. Click the Done button.