Ratio Image Action Options

Use the Ratio Image action to set the ratio image parameters for an experiment. A ratio image is basically a method of viewing experiment status.

1. From the Actions tab on the Design tab, select Ratio Image as the action and then select Options. The Ratio Image Action Options window is displayed.

2.  To use Channel 1 as the ratio’s numerator, activate the Use Channel 1 as Numerator checkbox.

3. In the Channel 1 and Channel 2 Background fields, enter the intensity values to subtract from each channel.

4. Click the Done button.

While a ratio imaging experiment is running, a two-channel time-lapse image is displayed along with a ratio graph showing the mean value vs. time. The mean value for a ratio graph is calculated using a specified portion of the image.