Use the UltimateFocus action to set up UltimateFocus parameters for a time-lapse experiment.
From the Actions tab on the Design tab, select UltimateFocus as the action and then select Options. The UltimateFocus Action Options window is displayed.
In the Move Threshold field, enter the desired maximum measured focus error (in um) before a corrective action should be taken.
In the Maximum Iterations field, enter the maximum measure/move sequences for this action to reach the calibrated focus point.
Click the Done button.
Note: More iterations will take longer, but may provide better focus.. |