Run Experiment Options

Note:  Experiment macros are "scripts" of commands that guide the microscope to collect images. These macros should be designed after you have manually acquired images of your specimen and adjusted exposure and other parameters until you are satisfied with the images.

Use

To...

Image file name

Enter the file name for the image. If you do not provide a file name, you will be asked to provide it when the experiment starts running.

Note:  If you select Auto-increment file names in the Settings window, you will need to provide a name only once for each session. This option creates new file names by appending numbers to the original name.

Settings

Open the Resolve3D Settings window that lets you specify the current Data folder and Experiment macros folder (directory), and select the Auto-increment file names.

DMS Setup

Open the DMS Destination window to determine whether to upload files to the DMS database after the data is collected and to select the destination parameters for project, dataset, category group, and category. You can also provide an initial annotation from this window.

Image title

Enter the title. (The title is saved in the header of the image file created by the experiment and can be viewed later by choosing File | Get Info on the Image window and then clicking Header Labels in the Image Information window.)

Add note to log

Insert a note in the experiment log file before or while the experiment is running. Click Do It at the point you'd like to insert the note.

Change next time lapse

Change the next time-lapse value while the experiment is executing. Type the desired time (in seconds) in the text field and click Do It when you want to change the value. This value only affects experiment macros that contain a TLAPSE command. It does not permanently change the macro.

Note:  This option is not available when you are using Fast Acquisition.

Show images during acquisition

Display images in a Data Collection window as they are being collected. You can increase performance by deselecting this option.

Show PK progress graph

Display a real-time graph of intensities (measured at PK Event locations) vs. time during a photokinetics experiment.

Launch viewer after experiment

Launch an Image Window when the experiment is finished. If more than two files are created in a point-visiting experiment, the Image Windows will not launch even if this option is selected.

Deconvolve during experiment

Deconvolve the image during the data acquisition experiment.

Show intensity graph

Select whether to display an intensity graph and specify either Maximum Intensity or Mean Intensity.

Enable Post-Acquisition Processing

Set up specific processing tasks to occur after the images have been acquired. You can choose between having the post-acquisition tasks run automatically, immediately after the image acquisition is completed, or to simply run the tasks manually using softWoRx Queue Manager to activate the queue.

Images acquired / requested:

View the current number of images acquired, compared to the total number requested. 

Note:  During fast acquisition, the reported number of acquired images may not be updated regularly.

Disk space required

View the amount of disk space needed to perform the specified experiment.

Current command

View each macro command as it is executed.

Start Time

View the time that the experiment starts.

Current Time

View the current time (for reference).

Elapsed Time

View the elapsed time of the experiment, in seconds.

Estimated Finish

View the estimated clock time in which a running time-lapse experiment will finish.

Start Scan

Using the green arrow button at the top of the window, start the selected macro to run an experiment.

Cancel Scan

Using the red circle button at the top of the window, terminate a scan while the experiment is running. (The images that are collected before the experiment is cancelled are automatically saved.)