Use the Design tab on the Design/Run Experiment window to generate experiment macros. The options on this tab allow you to include Z sectioning, multiple wavelengths, time lapse, point visiting, and panel collection in the experiment.
Note: Experiment macros should be designed after you have manually acquired images of your specimen and adjusted exposure and other parameters until you are satisfied with the images. |
Open the Design/Run Experiment window by clicking the Experiment
icon on the Resolve3D window. Then click the Design
tab and the Standard sub-tab.
Field, Tab, or Menu |
Description... |
File Menu |
Includes options for saving and quitting the Experiment Designer. |
Experiment name |
Allows you to provide a specific experiment name. The .exp file extension is added to the name.
Note: If you do not enter a name in this field, the default name is used. softWoRx overwrites the default name without displaying a warning message. If you enter a specific name, a warning message is displayed before that experiment macro is overwritten. |
Estimated File Size |
Displays the estimated file size of the data files. |
Use Fast Acquisition |
Selects Fast Acquisition. |
Fast Acquisition Options
|
Opens the Fast Acquisition Options window that allows you to change the imaging order and camera shuttering. (You can scan through all of the Z sections and then switch the wavelength to eliminate time lost in changing filters.) |
Sectioning |
Allows you to stage 3D experiments. This involves moving the microscope vertically to change the focal plane of imaging in carefully controlled steps. |
Channels |
Allows you to choose the exposure, filters, and shutters for a single or multi-channel experiment and to create a reference image. |
Time-lapse |
Allows you to set up a time-lapse experiment. |
Points |
Allows you to set up a point visiting experiment. (Point visiting is used to monitor multiple points of interest in one session.) |
Panels |
Allows you to set up a panel collection macro. This is useful when you want to scan a large area with a relatively high magnification lens. |
Plate |
With the optional Microtiter Stage installed, a DeltaVision system has the added ability to scan microtiter plates. The Plate tab provides the additional tools necessary for designing and running microtiter plate acquisitions. |
Actions |
Allows you to specify certain actions to be performed conditionally during a time point or point visiting experiment. The available actions include Laser Event, UltimateFocus, Autofocus, Cell Track, Pause, Wait, Change Time Lapse, Ratio Image, Add Log Note, Trigger TTL, and Custom. |
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