Design Experiment Options

Use the Design tab on the Design/Run Experiment window to generate experiment macros. The options on this tab allow you to include Z sectioning, multiple wavelengths, time lapse, point visiting, and panel collection in the experiment.

 

Note:  Experiment macros should be designed after you have manually acquired images of your specimen and adjusted exposure and other parameters until you are satisfied with the images.

 

Open the Design/Run Experiment window by clicking the Experiment Icon_Experiment.jpg icon on the Resolve3D window. Then click the Design tab and the Standard sub-tab.

Field, Tab, or Menu

Description...

File Menu

Includes options for saving and quitting the Experiment Designer.

Experiment name

Allows you to provide a specific experiment name. The .exp file extension is added to the name.

 

Note:  If you do not enter a name in this field, the default name is used. softWoRx overwrites  the default name without displaying a warning message. If you enter a specific name, a warning message is displayed before that experiment macro is overwritten.

Estimated File Size

Displays the estimated file size of the data files.

Use Fast Acquisition

Selects Fast Acquisition.

Fast Acquisition Options

 

Opens the Fast Acquisition Options window that allows you to change the imaging order and camera shuttering. (You can scan through all of the Z sections and then switch the wavelength to eliminate time lost in changing filters.)

Sectioning

Allows you to stage 3D experiments. This involves moving the microscope vertically to change the focal plane of imaging in carefully controlled steps.

Channels

Allows you to choose the exposure, filters, and shutters for a single or multi-channel experiment and to create a reference image.

Time-lapse

Allows you to set up a time-lapse experiment.

Points

Allows you to set up a point visiting experiment. (Point visiting is used to monitor multiple points of interest in one session.)

Panels

Allows you to set up a panel collection macro. This is useful when you want to scan a large area with a relatively high magnification lens.

Plate

With the optional Microtiter Stage installed, a DeltaVision system has the added ability to scan microtiter plates. The Plate tab provides the additional tools necessary for designing and running microtiter plate acquisitions.

Actions

Allows you to specify certain actions to be performed conditionally during a time point or point visiting experiment. The available actions include Laser Event, UltimateFocus, Autofocus, Cell Track, Pause, Wait, Change Time Lapse, Ratio Image, Add Log Note, Trigger TTL, and Custom.