Point Visiting

Use Point Visiting to monitor multiple points of interest in a single session. This is especially useful for live cell experiments. You can save sample preparation time by capturing all of the data required for your experiment from a single sample in one session instead of acquiring the data from multiple samples.

Use the following process to set up Point Visiting:

 

 

Mark Points - Use the keypad to quickly mark the approximate point locations. Then use Resolve3D to refine the Z coordinates for these locations.

Save Point List - Edit and Save the list of points.

Set up Experiment - Create a Point Visiting macro and specify which points to visit in the Design/Run Experiment window.

When you run the experiment, DeltaVision reads the coordinates for the points in the list, moves the stage to each point, and captures an image. This process is repeated at specified time-lapse intervals.

Like other macros, Point Visiting experiments should be designed after you have manually acquired images of your specimen and adjusted exposure and other parameters until you are satisfied with the images.

Notes:

  • If you are placing your slide directly on the microscope stage, you can store the X, Y, and Z coordinates of the stage positions you mark; however, if the slide is removed from the stage or the Z focus knob is adjusted, these coordinates no longer apply.

  • If you are using GE Healthcare’s Repeatable Slide Holder, you can remove the slide and then place it in the same position when you want to revisit the points. To do this, you must record the position (A-G) of the slide before you remove it. Then put the slide in the exact same position when you return it to the slide holder and update the Z coordinates.