Localization microscopy data may be visualized in various ways, including by reconstructing a super-resolution image. Localization image reconstruction involves using the detected fluorophore positions to generate an image with the desired pixel size and fluorophore representation.
It is in the reconstruction phase that the user can decide to plot all appearances of each fluorophore, or group the appearances into a single x- and y-coordinate. Deciding to group the appearances, also known as plotting tracks, can only be done if the user has completed the tracking phase. If the user decides to plot tracks, the reconstruction algorithm will determine a new position for the fluorophore as the average of its multiple appearances, where each appearance is weighted by its localization precision. A new localization precision is determined based on the sum of the detected photons for each frame in which the fluorophore appears.
Setting Up Image Reconstruction