If you determine that you want to re-scan some of the wells (due to Autofocus miscalculations, etc.), you can re-scan an individual well or group of wells using the same experiment macro. The difference is that you’ll be modifying the Plate Point List to select only the well(s) to be re-scanned.
To rescan selected wells:
1. From the Plate View window, select the well or wells to be re-scanned and click Generate Plate Point List to replace the current Marked Point list with this modified one. Remember, that the points are generated with the current stage Z value, so make sure the DeltaVision system is in focus.
2. In the Design/Run Experiment window, use the same experiment name as you used the first time. You may be prompted to re-save the experiment since the point list has changed.
3. From the Design/Run
Experiment window, click the Run
icon to restart the scan. You are prompted to confirm
that you want to overwrite the file(s) you scanned before.