Save As TIFF Options

Use the Save As TIFF window to save the DeltaVision image that you're viewing as a series of TIFF (Tagged Image File Format) files. You can choose to save the data as 8 or 16 bit single-wavelength TIFF files or as 24-bit (8 bits per wavelength) RGB color TIFF files. You can use the settings that are currently being used to view the data to scale these images, or you can save them unscaled.

Open the Save As TIFF window by choosing File | Save As TIFF from the Image window.

Notes

  • The Save To TIFF utility does not generate "What You See Is What You Get" images. You will not see scale bars and other ancillary graphics in the TIFF files. The utility is intended primarily for exporting image data in a standard and generally-supported format for further data processing. To save a picture from the screen for presentation purposes, it is recommended that you use the Save As Photoshop option in this menu or the Image Snapshot option in the softWoRx Utilities menu.

  • When saving an image file that has multiple wavelengths, multiple Z sections, or multiple time points, softWoRx automatically generates file names for the series of frame files. If you plan to save and use these files on a DOS computer, specify Short File Names to eliminate information such as the wavelengths, Z section, and time point from the file name. (Short file names are more cryptic.)

Use

To...

Input, Output, Select region, Reset, Details,

Wavelengths

Specify input data and output parameters as shown in Image Input and Output.

Output Directory

Specify the output directory for the TIFF files. The default is the directory that is specified in the Input field.

File Prefix

Provide a string from which file names for the movie and frame files are created. The default value is the same as the input image.

Note If you select Short File Names, try to use just one or two characters for the Prefix.

Scale Using Min/Max/Exp Values Below

Scale data using the current display scaling factors for the full dynamic range of the output data samples.

 

For example, if you have 16-bit data that is being viewed using 100 as the minimum value and 6000 as the maximum, the output 16-bit data file will map all values 100 or less to 0 in the output file and all values equal to or greater than 6000 to the largest integer a 16-bit value can hold (65534). All values in between the minimum and maximum are interpolated to the appropriate values.

 

Note The option of scaling is disabled under certain conditions. If the output format is set to 16-bit Gray, the data will be saved unscaled.

Compression

Specify a compression scheme for the TIFF files. Be certain that the software package that you are using to view or analyze the TIFF files supports compressed files.

Short File Names

Generate files with DOS-compliant file names (of the form XXXXXXXX.XXX). If you supply too many characters in the File Prefix field, a warning message is displayed.

Destination Computer

Swap the order of the bytes that make up each 2-byte (16-bit) sample. Choose from: Windows PC or Linux (the default) or  Macintosh or SGI. (You may need to do this for 16-bit images to be read by some programs on IBM-Compatible PCs.)  

Output Size

Specify the size of each sample in the output data file. Many softWoRx data files are 16-bit integer files, so you may lose dynamic range if you put 16-bits of data in 8-bit (or 24-bit, which has 8 bits per wavelength) files. However, not all software packages support 16-bit grayscale or 24-bit color TIFF files.

Color

 

Assign colors. If you have set the Output Size at 24-bits, you can assign Red, Green, or Blue to each wavelength (up to 3 wavelengths).

Min/Max/Exp

Scale the output data according to the values in the Minimum, Maximum, and Exponent fields. The data is scaled before it is converted to the specified output data format.