To set up the Localization Image Reconstruction tool:
1. From the softWoRx main menu bar navigate to Measure | Localization Image Reconstruction. The Localization Image Reconstruction window is displayed.
2. Click the Input button to browse to and select the DeltaVision image file to reconstruct. Alternatively, the user can click and drag an image window number or a file from the data folder into this field.
Note: If opened from the Localization Image Analysis | Reconstruction tab, the window pre-populates with the file name of the generated super-resolution DeltaVision file (*_SRR.dv) and the localization text file from the previous Reconstruction. |
3. Click the Localization Data button to browse for and select the localization file to reconstruct (*_LOC.txt or *_LDC.txt). Alternatively, you can click and drag a file from the data folder into this field.
4. Define an ROI that will be used to generate a super-resolution reconstruction. If an image window is the input, you can use the Select Region window to draw a box around the ROI. Otherwise, use the Details button to define the x and y starting coordinates and width/height in units of pixels. The Select Region window automatically populates the Details window with the coordinates of the drawn ROI.
The Localization Image Reconstruction Tool
Setting Up Image Reconstruction