Setting Up the Multiplexed Wavelength Option

Before you begin designing your Multiplexed Wavelength experiment, you’ll need to perform the steps described in the following procedures to activate a Multiplexed Wavelength filter set and prepare the DeltaVision system for Multiplexed Wavelength operation.

To activate the Multiplexed Wavelength filter set:

  1. To change the active filter set to a filter set that is Multiplex capable, select Settings in the Resolve3D main menu. The Resolve3D Settings window is displayed.

  2. In the Resolve3D Settings window, click on the Misc tab.

  3. In the Resolve3D Settings window, select the EX and EM filter sets you want to use. When these fields are changed, the <<<Pending Activation message is displayed in the window.

  4. Click Activate Filter Sets. A confirmation window is displayed.

Notes:  
1) If you select filter sets for the Excitation filter wheel and Emission filter wheel fields and then click Done in this window, your selections are retained until you either activate the filter sets or exit Resolve3D.
2) For the filter sets to be activated, the selected multiplexed filter set filters must exist in the currently installed excitation and emission filter wheels.

  1. If the selected filter wheels are installed on your DeltaVision, click Next to continue.

Note:  If you click Skip from this window, the selected filter wheels are activated immediately and the remainder of the activation wizard is skipped.

  1. Insert the selected secondary filter insert into Filter Slot 1 of the secondary light path and click Next to continue. The system gathers the information for this window from the MXWSetup.ini file, not from the Instrument Controller.

  1. Move the beam combiner to the appropriate position and click Next to continue.
    Again, softWoRx gets the information for this window from the MXWSetup.ini file, not from the Instrument Controller.

  2. Click Finish to complete the Multiplexed Wavelength filter activation process.

At this point, you have completed activating the Multiplex Wavelength filter set. You should now continue with the procedure for viewing a sample with the Multiplexed Wavelength operation.