- For time-lapse intervals nearly the same or less than the imaging time requirement.
When imaging as fast as possible, it seems very unlikely that significant enough environmental drift would occur from one frame to the next to make a discernible difference in focus. Therefore, to maximize frame rate, you can easily design an experiment that triggers an UltimateFocus Action with a certain periodicity. For example, you can choose to do UltimateFocus maintenance every 20 frames. You would need to toggle the Maintain Focus with UltimateFocus button Off in the Time-lapse tab of the Experiment Designer and add an UltimateFocus Action with every 20 as the specification for which time points to apply the Action. This allows fairly fast imaging (as fast as conventional time lapse can do), but with an occasional UltimateFocus maintenance event to compensate for drift.