Use
To...
Center Object
Center the stage on an object selected in an Image Window.
Note The pixel size must be correct in order for object centering to work properly, which means that the correct lens and auxiliary magnification setting must be selected. An image is acquired after the object is centered.
Mark Point
Mark the current X, Y, Z stage coordinate as a point to be visited later.
Mark Top of Sample
Mark the current stage Z position as the top of your sample. Because the scanning process moves the stage toward the objective, this position also represents the point where the stage is closest to the objective (the most negative Z value).
Use this along with the 
button to
establish the thickness of the sample you're imaging. You can use these
marked positions to aid with the Z
sectioning setup in the Experiment Designer by choosing the Get Thickness
button.
Note Scans set up using the Experiment Designer all scan in Z using relative coordinates. The Mark Top and Mark Bottom buttons are most helpful in determining the thickness of the sample to be scanned. When an experiment is started, the scan region is determined by the current focus point and the thickness of the sample. So, for example, if you have marked three points to visit in an experiment and they all have different middle-Z positions, the experiment calculates the scan based on these different Z positions and the fixed thickness.
Visit Top
Move the stage to the top-of-sample position (If this position is marked).
Mark Bottom of Sample
Mark the current stage Z position as the bottom of your sample for a potential scan. This corresponds to the positive stage Z coordinate value.
Visit Bottom
Move the stage to the bottom-of-sample position (if this position is marked).
Visit Middle
Visit the point in the middle of the defined scan region.
Microtiter Plate
Display the Microtiter Plate Viewer. The Microtiter Stage is optional on a DeltaVision system.
AutoFocus
Automatically Focus.
Acquire Image
Collects and displays an image from the microscope. The current settings are used to collect the image. This image is only displayed in the Data Collection window. It is not saved in a disk file for later use.
XY Stage Motion
Controls
Move the stage in the X and Y axis. The left and right arrows move the stage in the X axis in the increment set in the dX field and the up and down arrows move it in the Y direction in the increment set in the dY field.
Z Stage Motion Controls
Move the stage in the Z axis in the increment set in the dZ field.
Pan
Move the stage view. This tool is "sticky", so to disable it, click the Pan icon again.
Spiral Mosaic
Starts a preview collection pattern centered on the current stage location and using the active Resolve3D wavelengths. The pattern begins with the current stage location and then continues acquiring thumbnail images directly adjacent to it, spiraling outward in a counter-clockwise rotation so that the entire area centered around the initial stage position is previewed in the Resolve3D stage view. Thumbnail collection continues until either the preset spiral mosaic size (set in Resolve3D | Settings | Misc tab) is reached or you click the Spiral Mosaic button again.
Clear Stage Trails
Clear all of the stage-trail lines from the stage view.
Clear Thumbnails
Clear all of the thumbnail images that are displayed on the stage view.
Marked Points List
Open the Point List window to manage the list of marked points.
Zoom Thumbwheel
Control zoom. Drag the thumbwheel down to zoom in; up to zoom out.
The button directly beneath the zoom thumbwheel returns the zoom to 1.0.
Z Slider
Moves the stage up or down. As the mouse is released, an image is acquired.