To stitch an image:

1. From the softWoRx main menu, click View | Stitch to open the Stitch Image window.

2. Click Input and browse to the file that you want to stitch. softWoRx reads the wavelengths from the file header and automatically selects the appropriate wavelengths. If your application does not require a stitched file for a particular wavelength, deselect that wavelength.

3. Click Output and browse to the same file previously selected. The file will appear in the Output field with “_STC” added to the file name. (With this naming convention, the input source file is always associated with the output stitch file.)

4. To display additional stitch options, click More Options. Additional options include settings for adjusting image geometry, settings for intensity scaling, and settings for adjusting the stage polarity. For most applications, the default settings should work quite well. When finished with this window, click Done to return to the main Stitch Image window.

5. When all of the options are specified, click Do It.