Open
the Localization Precision Histogram window by clicking the Show
Localization Precision Histogram button in the Localization
Image Analysis | Reconstruction tab window.The Localization Precision Histogram tool is designed to help the user choose the minimum and maximum localization precisions used for super-resolution image reconstruction.
Open
the Localization Precision Histogram window by clicking the Show
Localization Precision Histogram button in the Localization
Image Analysis | Reconstruction tab window.
The Localization Precision Histogram window shows a histogram of the localization precisions for the detected fluorophores in the input localization text file. The numerical values at the top of the histogram display the minimum localization precision (Min), the maximum localization precision (Max), and the mean localization precision (Mean) including the standard deviation for the entire data set, all in units of nm. Additionally, the header displays the user-defined minimum and maximum localization precisions (in nm) to be plotted in the Reconstruction. The minimum and maximum localization precisions may be changed by dragging and dropping the Min and Max markers on the histogram plot. The final line of the header describes the total number of fluorophores to be plotted as a fraction and percentage of the total number of fluorophores in the input file. As the user changes the values for the Min and Max localization precisions, the values in the header are updated appropriately to show how the changes will affect the number of fluorophores used to generate the reconstruction. Ideally, the maximum localization precision will be chosen to maximize the number of detected fluorophores with high positional certainties that are used to generate the super-resolution reconstruction.