After running the localization analysis tool, the results of the selected parameters can be evaluated by comparing the detected fluorophore positions to the original acquired image. Depending on the results, the user may elect to change localization parameters. The Localization Results Viewer can be used to verify the settings for the localization analysis by overlaying the detected fluorophore positions on the original acquired image.
Since the localization file contains sub-pixel coordinates, the detected fluorophore position is plotted on the original image pixel coordinate corresponding to where the fluorophore was detected. At the bottom of the Localization Results Viewer, the number of currently plotted points is displayed and updates according to the viewing style selected.
Using the Localization Results Viewer