To remove data below an intensity threshold:

1. Choose Filter | Threshold from the softWoRx main menu to open the Threshold window.

2. In the Input field, enter an image file name or window number.

3. To include only selected data, click Details to open the Region Details window. Then specify the ranges of data that you want to include in the Output Options fields.

4. Select which wavelengths to filter.

5. In the Create Mode option list, select one of the following options:

   Choose Data to create an output image of all intensities equal to or above the threshold (the cutoff intensity value). If a pixel has an intensity value below the threshold, the pixel in the output image is set to this value (usually 0).
   -or-
   Choose Mask to put zeros in all pixels where the intensity is below the threshold and ones for all pixels equal to or above the threshold.

6.  Specify a Threshold and Background value for each wavelength.

7. Click Do It.