Use the Time-lapse tab fields to specify the number of time points, the time periods, and the total time for a time-lapse experiment. (The Time-lapse tab is under the Design tab in the Design/Run Experiment window.) Like other macros, Time-lapse experiments should be designed after you have manually acquired images of your specimen and adjusted exposure and other parameters until you are satisfied with the images.
Note: You'll need to specify only two of the three fields in this section. After you specify these fields, softWoRx calculates a value for the third field. In most cases, you'll enter a value in the Time-lapse field and then enter a value in either the Time Points or Total Time field. |
Use |
To... |
Time-lapse (check box) |
Activate the check box to enable a time-lapse experiment. |
Time-lapse |
Specify the hours, minutes, and seconds for each time period. |
Total Time |
Displays the total time of the experiment, which can also be calculated as follows: Total Time = (Number of time points - 1) x Time-lapse |
Time Points |
Specify the number of time samples to collect in a time-lapse experiment. |
Enable Cell Tracking |
Move the stage laterally to follow cells as they move during a time-lapse experiment. With the Enable Cell Tracking option selected, DeltaVision automatically keeps cells in the field of view. |
Enable Cell Tracking (check box) |
Moves the stage laterally to follow cells as they move during a time-lapse experiment. With this check box activated, DeltaVision automatically keeps cells in the field of view. |
Cell Tracking Options |
Opens the Cell Tracking Options window that allows you to specify the parameters for cell tracking. |
Maintain Focus with UltimateFocus (check box) |
Specifies focus should be maintained during the time-lapse experiment. If this experiment does not involve point visiting, a calibration (characterizing the UltimateFocus response at the current location) is performed when the experiment is started. Notes: #1 The initial focus is essential to the success of focus maintenance. #2 The actual focus maintenance process happens just before each time-lapse event. |
UltimateFocus Options |
Opens the UltimateFocus Options window, in which you can indicate a specific Move Threshold and the Maximum (number of) Iterations to move within the threshold before re-running UltimateFocus. • In the Move Threshold field, enter the desired maximum measured focus error (in mm) before a corrective action should be taken. • In the Maximum Iterations field, enter the maximum measure/move sequences for this action to reach the calibrated focus point. |
Image-based Autofocus before imaging (check box) |
Automatically focus the camera before acquiring each time point. (This option does not cause DeltaVision to AutoFocus between wavelengths or Z steps.) For point visiting experiments, the camera is automatically focused every time a point is visited. |
Autofocus Options |
Opens the Resolve3D Settings window to the Autofocus tab, in which you can manipulate the settings for the software-based Autofocus function. |
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