Set the Beam Selector to 
(Eye on
some systems).
Select the eyepiece filter by rotating the eyepiece filter wheel below the oculars on the scope.
You will see your selection in the Filter Monitor window on the right-hand side of the workstation screen. As you rotate the filter wheel, the filter name next to EP (eyepiece) changes and the EM and EX (emission and excitation) filters change automatically to match. The displayed colors match the wavelength of the filters. (If your system does not have the Reporting eyepiece filter wheel, the EP filter name is not displayed. On a manual eyepiece filter wheel, filters can be identified by raised metal marks on the outside of the filter wheel. )
Open the shutter by pressing the EX SHUTTER button in the lower left corner of the keypad. You should see light through the objective.
Find your specimen by moving the stage with the joystick. Focus using the coarse and fine focus knobs on the scope body (see To find and center a Specimen).
With
your sample centered and focused through the oculars, close the shutter
and rotate the Beam Selector to the 
position.
(On some systems, this is labeled SPL
or SP.) This directs the light path to the camera rather than
the oculars.
Enter an exposure time in seconds in the Resolve3D window Exposure field. A good starting exposure time is 0.1 second (see Finding Exposure Time).
Note: You can also find the appropriate exposure time by clicking the Find button. Use Find carefully. This option can photo-bleach the specimen. |
Click the Acquire button to acquire an image. The image is displayed in the Data Collection window.
Adjust the image to avoid saturation and get good signal-to-noise ratio. Pixel intensity values range from 0 to 4095. (Minimum and maximum intensity values for your image are displayed next to the Min and Max labels near the bottom of the Resolve3D window.)
Use
the position buttons in the middle of the Resolve3D window to find
and center the object of interest. Use the Z Slider on the right side
of the Resolve3D window to find and center the object of interest.
Drag the slider up to find the top of your sample. (When you release
the mouse button, an image is acquired and displayed in the Data Collection
window.) Drag the slider and acquire images until you are satisfied.
Then press the 
button
to mark that location. Next, use the slider to find the bottom of
the sample. When you are satisfied, press the 
button
to mark that location.
The top and bottom can be viewed by pressing the
button or
the 
button
respectively. The middle section can be viewed by pressing the 
button.
Notes:
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