To analyze Photokinetic data:

  1. From the main softWoRx menu, choose Measure | PK Analysis to open the Photokinetic Data Analysis window.

  2. If you are analyzing a multipoint Photokinetic data set, enter the number of bleached points in the Number of Laser Sites field.

  3. Click Do It to run the analysis. The following files are generated:

    4. A three channel output image file that includes the original time lapse image, the ratio of the current time point to average pre-bleach time-points, and the ratio data at the location used for analysis.

     

    A JPEG file that contains a recovery graph, that is a plot of the fluorescence intensity before and after the event.

     

    A log file (in a text format) that contains the analysis results.

 

Tip You can use the Photokinetic Data Analysis window to choose how to curve fit the recovery data, specify the type of bleach pattern, shape, and number of sites. You can also use it to remove background intensity, select which Z sections and wavelengths to include in the analysis, and other options.