On the FRET Analysis window, choose the Analyze Results tab.
Make sure that the FRET Results image that you want to analyze is open in an image window.
In the FRET Results Window field, make sure that the window number of the FRET Results image window is specified.
Use the ROI creation tool icons at the top of the FRET Analysis window to specify one or more regions of interest.
If you have an image with multiple time points or multiple Z sections, you may want to propagate ROI polygons through Time or Z. To do this, create a polygon, make sure it is selected, then choose Copy Through Time or Copy Through Z to propagate the polygons. (As changes to ROI polygons are made, the table of statistics is updated to reflect the statistics of the chosen ROI set.)
To export the table of numbers in a form that can be used in a spreadsheet, choose Save Results As SYLK (Symbolic Link format) or Save Results As CSV (Comma-Separated Values) from the Fret Analysis File menu.
To view the results in a graph, click Plot Results to generate an X/Y plot of parameters that you choose. If the X axis is time-related, the software associates ROIs from time point to time point and plots the values as connected sets on the graph.
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Tips #1 You can modify the details of how the graph is displayed once it is created with the Graph-Properties tool. #2 You can optionally save the FRET Results graph by selecting Save As JPEG from the FRET Results graph's File menu. |