To automatically track cell movement:

Cell Tracking moves the stage laterally to follow cells as they move during a time-lapse experiment. With the Enable Cell Tracking option selected, DeltaVision automatically keeps cells in the field of view.

  1. Set up a time-lapse experiment.

  2. On the Time-lapse tab on the Design/Run Experiment window, select Enable Cell Tracking.

  3. Click Cell Tracking Options.

  4. In the Reference Channel list, specify the channel to use for pattern recognition. (The channels are numbered in the order that they are listed on the Channels tab.)

  5. In the Move Threshold field, enter the distance in microns that the cell must move to trigger stage movement.

  6. Use the ROI % slider to specify the area around the cell (the region of interest or ROI) that you want DeltaVision to use for pattern recognition.

  7. If you are performing an experiment on a single cell, use the stage controls to center the cell (laterally) in the field of view. If you are performing a point visiting experiment, make sure that the points that you are monitoring are centered before you start your experiment.

  8. Click the Run tab and then click the green arrow Run_icon_sm.JPG button at the top of the window to run the experiment.

Note:  Cell Tracking is available only for DeltaVision systems.

Related Topics

Cell Tracking Options