To collect 3D panel images:

Before you collect panel images, you must first determine the number of voxels in the Border Rolloff that is used in Deconvolution.  

  1. Determine the size of the panels that you plan to collect. (Smaller panels work best because they have the flattest intensity distribution.)  

  2. From the main softWoRx window, choose Process | Deconvolve to open the Deconvolve window. In the Input field, enter the file name of an image that is similar to the panel size. (You can acquire and save a blank image of that size for this purpose.)

  3. Click the More Options button to open the More Deconvolution Options window and record the value displayed in the Border Rolloff (voxels) field.

  4. From the Resolve3D window menu, choose View | Point List. In the Point List window, choose Mark Point and mark points in two opposite corners to define the area of your final composite image.

  5. In the Resolve3D window, click the Experiment Icon_Experiment.jpg icon to open the Design/Run Experiment window.   

  6. Click the Design tab. Then set up the Sectioning and Channels information in the same way that you would for standard data collection.

  7. In the Point List window, select one of the points that you marked in Step 4 and click Visit Point.

  8. Click the Panels tab under the Design tab. Then, select the Collect Panels option.

  9. Click Get Start under the Panels tab. Then visit the other point that you marked in Step 4 and click Get End.  (Alternatively, you can enter the coordinates that are displayed in the Point List window.)

  10. In the Overlap (pixels) field, enter at least twice the number of border rolloff voxels as you recorded in Step 3. If you generally adjust the pixel size to <1 or use rotation when stitching, use a larger overlap. (The spacing information is automatically updated.)

  11. Choose File | Save As and enter the name of the macro.

  12. Click the Run tab. Then, click the Start Scan Run_icon_sm.JPG button.

  13. When data collection is finished, choose File | Close Window in the Design/Run Experiment window.