To combine data of two image files:

  1. From the main softWoRx menu, choose View | Image Fusion to open the Image Fusion window.

  2. In the Image 1 and Image 2 boxes, enter image file names or window numbers for the two files that you want to combine.

  3. In Image 1 Selections, select which wavelengths, time points, and Z sections to combine from the first image.

  4. In Image 2 Selections, select which wavelengths, time points, and Z sections  to combine from the second image.

  5. Under Fusion Options, specify how to combine the data as follows:

    To append all selected wavelengths to the output data set, choose Append Wavelengths  (With this option, if Image 1 had wave 490 selected and Image 2 had wave 490 selected, the output data set would have two separate 490 wavelengths.)
    -or-
    To combine timepoints from all selected matching wavelengths into a single final wavelength data set, choose Combine timepoints for like wavelengths.
    -or-
    To combine Z sections from all selected matching wavelengths into a single final wavelength data set, choose Combine z-sections for like wavelengths.

  6. Click Do It.

Notes

  •  If error messages are displayed that refer to differences in file types that include image size, pixel size, lens info, data type, etc., use Copy Region and Edit Header to manipulate these items.

  •  If the number of sections varies from wavelength to wavelength during an operation,  Blank Z sections have been added to the image is displayed. These blank sections are added to balance the number of sections between each wavelength of the output image.  Each one is a zero intensity image added to the end of the appropriate wavelength.