To design a time-lapse experiment:

Manually acquire images of your specimen and adjust settings (e.g., exposure time) until you are satisfied with the images.
Select the Time-lapse option in the Design/Run Experiment window, activate the Time-lapse check box, and enter the desired time interval in the Time-lapse field. (The minimum time interval is limited by acquisition time. If you specify a time that is less than the acquisition time, the experiment proceeds at the fastest possible rate.)
Set up the remaining parameters for your experiment in one of the following ways:

Enter the desired number of time points in the Time Points field. The Total Time field displays the total time that will elapse during the experiment.

-or-

Enter the total time in the Total Time field. The Time Points field displays the number of time points for the experiment.
Click the Run tab and save the changes to your experiment.
Enter a file name in the Image file name field and enter a descriptive title in Image title. Then activate the Show Images during acquisition check box.
Click the Start Scan button. The images are displayed in the Image window as they are collected.