To find and center the specimen:

1. Secure the slide on the stage. Since DeltaVision is an inverted microscope, the sample must be placed with the cover slip facing down toward the objective. If you are using a standard microscope slide (1” x 3” or 25 mm x 75 mm) and you have the Repeatable Slide Holder, you can use it to hold the slide.

2. Choose the objective by rotating the microscope nosepiece. Be sure to select the same objective in the Resolve3D window.

3. Choose the desired eyepiece filter.

4. Select the desired excitation, neutral density, and emission filters in the Resolve3D window. (If you are using the Reporting Eyepiece Filter Wheel, these parameters should automatically be in place after selecting the eyepiece filter, unless non-standard arrangements are needed.)

5. Rotate the Beam Selector at the base of the microscope to direct the light collected by the objective to the eyepiece.

6. If using the transmitted light to locate the specimen, open the transmitted light shutter by pressing TRANS SHUTTER on the keypad. This allows light to impinge on the sample. (The power switch for the transmitted light is located on the microscope base. The dimmer is located below the power switch.)

7. Set the stage speed using the keypad.

8. Find the desired focal plane optically using either the focus knobs on the microscope or the Z stage controls. Use the keypad to maneuver the slide to find an area of interest.

9. Open the EX shutter using the keypad and find the proper focal plane using the microscope focusing knob. Tighten the focusing tension ring.

10. Use the keypad or fine focus knob to find the approximate center of the specimen and place it in the center of the field of view using stage controls.

11. Direct light to the camera port using the knob on the microscope base.